Lab 6 enzymes answers

  • Special enzymes termed restriction enzymes have been discovered in many different bacteria and other single-celled organisms. These restriction enzymes are able to scan along a length of DNA looking for a particular sequence of bases that they recognize. This recognition site or sequence is generally from 4 to 6 base pairs in length.
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Biology Lab Notebook Table of Contents: 1. General Lab Template 2. Lab Report Grading Rubric 3. Sample Lab Report 4. Graphing Lab 5. Personal Experiment 6. Enzymes Lab 7. The Importance of Water 8. Cell Membranes - How Do Small Materials Enter Cells? 9. Osmosis - Elodea Lab 10. Respiration - Yeast Lab 11. Cell Division - Egg Lab 12.

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  • Ask your lab instructor whether your group will do III temperature or IV pH. III. Effect of Temperature on Enzyme Activity. Enzymes are designed to function within a specific temperature range. You might make some guesses as to the outcome for the following temperatures below.
  • (A) the reaction is thermodynamically impossible at pH 6.0 (B) the enzyme is not active at this pH (C) a pH of 6.0 prevents color development beyond an absorbance of .38 (D) the enzyme is degraded more rapidly at pH 6.0 than it is at pH 5.0 (E) most of the substrate was digested during the first 10 minutes (1990 #115) 5.

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    laws of thermodynamics (revised 6/20/01) reactions and enzymes (revised 6/20/01) atp and biological energy (revised 3/12/07) cellular metabolism and fermentation (revised 3/12/07) photosynthesis (revised 6/24/01) introduction to genetics (revised 6/21/01) gene interactions (revised 6/21/01)

    Enzymes can act rapidly, as in the case of carbonic anhydrase (enzymes typically end in the -ase suffix), which causes the chemicals to react 107 times faster than without the enzyme present. Carbonic anhydrase speeds up the transfer of carbon dioxide from cells to the blood.

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    5. T or F: (circle) Enzymes increase the rate of reaction. 6. T or F: (circle) Enzymes are not used up because they are reused over and over. 7. Why are enzymes called Biological Catalysts?_____ 8. Enzymes help _____the activation energy of a reaction, which allows the reaction to speed up.

    This lab measures the enzyme, in this case, lactase, specificity upon a certain substrate, which in this lab was lactose. Enzymes help in digesting food and by catalyzing reactions to lower the required amount of Explain your answer. - Digestion reaction, because it is literally the breaking down of the...

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    6. Below is a plasmid with restriction sites for BamHI and EcoRI. Several restriction digests were done using these two enzymes either alone or in combination. Use the figure to answer questions 4–6. Hint: Begin by determining the number and size of the fragments produced with each enzyme. "kb" stands for kilobases, or thousands of base pairs ...

    Enzymes are a protein serving as a catalyst, a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals, like the different players on a ball team, they have different specific structures and jobs.

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    References Witt, Wolfgang. "Purification and Properties of a Starch Granule-degrading α-amylase from Potato Tubers." Purification and Properties of Starch Granule-degrading A-amylase from Potato Tubers 47.304 (1996): 1789-795. Purification and Properties of a Starch

    Experimental Errors. It is impossible to make an exact measurement. Therefore, all experimental results are wrong. Just how wrong they are depends on the kinds of errors that were made in the experiment.

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    For every enzyme, there is an optimum pH value, at which the specific enzyme functions most actively. Any change in this pH significantly affects the enzyme activity and/or the rate of reaction. To know more about the relation between pH and enzymes, and/or the effect of pH on enzymes, go through this write-up.

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    Mar 09, 2008 · I did a lab last week but i'm confused about my data. The distance the disc travelled under hydrogen peroxide was equal every time. I began from 80°C which was NOT OPTIMAL temperature & cooled it down to 30°C. So, if the distance was same every time, should the time increase OR decrease as the temperature was decreased (Optimal temp. was unknown) & so what about the reaction rate?:S

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    Aug 06, 2020 · Plant Physiology ® is an international journal devoted to physiology, biochemistry, cellular and molecular biology, genetics, biophysics, and environmental biology of plants.

    7.5 Reporting Enzyme Activity Data / 257 7.6 Enzyme Stability / 258 7.7 Summary / 263 References and Further Reading / 263 8 Reversible Inhibitors 266 8.1 Equilibrium Treatment of Reversible Inhibition / 268 8.2 Modes of Reversible Inhibition / 270 8.3 Graphic Determination of Inhibitor Type / 273 8.4 Dose—Response Curves of Enzyme Inhibition ...

Oct 26, 2013 · 28 elevated liver ast enzyme of 67 at one lab, one week prior at another lab was 63. All other labs normal, no hepatitis of any kind. No pain Liver ultrasound showed no fatty liver or cancer. Not regu … read more
Biology I Lab instructions: Enzymes Lab. 5. Today's Lab • Investigating the enzyme catalase 2 H2O2 2 H2O + O2 • Occurs in peroxisomes in all cell types • In the reaction what is the substrate?
As more enzymes become involved in reactions, the rate of reaction increases. At some point near B , all the enzymes are being involved in reactions. When this happens, some of the substrate must "wait" for enzymes to clear their active sites before the enzyme can fit with them (like a "lock and key").
Oct 31, 2010 · Tube #6 (peroxidase enzyme) add the following: 2.0 mL of turnip peroxidase enzyme 3.0 mL of distilled water ... Laboratory reports should contain answers to the following questions: 1. What effect ...